All-In-One: Advanced preparation of Human Parenchymal and Non-Parenchymal Liver Cells
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چکیده
منابع مشابه
All-In-One: Advanced preparation of Human Parenchymal and Non-Parenchymal Liver Cells
BACKGROUND & AIMS Liver cells are key players in innate immunity. Thus, studying primary isolated liver cells is necessary for determining their role in liver physiology and pathophysiology. In particular, the quantity and quality of isolated cells are crucial to their function. Our aim was to isolate a large quantity of high-quality human parenchymal and non-parenchymal cells from a single liv...
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Recently, Pfeiffer and colleagues have published protocols that allow the isolation of human hepatocytes and non-parenchymal liver cells from the same donor (Pfeiffer et al., 2014). Cell isolation is performed with resected liver tissue, usually obtained from hepatectomy because of metastasis from colon cancer. Human liver cells are initially isolated by the conventional two-step EDTA/ collagen...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2015
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0138655